Characterisation of microbial community composition of a Siberian tundra soil by fluorescence in situ hybridisation
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چکیده
منابع مشابه
Characterisation of microbial community composition of a Siberian tundra soil by fluorescence in situ hybridisation.
The bacterial community composition of the active layer (0-45 cm) of a permafrost-affected tundra soil was analysed by fluorescence in situ hybridisation (FISH). Arctic tundra soils contain large amounts of organic carbon, accumulated in thick soil layers and are known as a major sink of atmospheric CO(2). These soils are totally frozen throughout the year and only a thin active layer is unfroz...
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The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belo...
متن کاملPhylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA.
Genomic DNA was isolated from the active layer of tundra soil collected from the Kolyma lowland, Northeast Eurasia, near the Arctic Ocean coast. The SSU (small subunit) rRNA genes were amplified with eubacterial primers from the bulk genomic community DNA and cloned into plasmid vectors. Forty-three SSU rDNA clones were obtained, and all of them had different RFLP patterns. Phylogenetic analysi...
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Aim-To determine whether the fluorescent in situ hybridisation technique (FISH) using a total human DNA genomic probe can be used to enumerate semen leucocytes.Methods-Semen samples from five donors were subjected to a mild KC1 solution. These samples were then biotin labelled under FISH conditions using a total human DNA genomic probe and the leucocyte counts were determined. To check the accu...
متن کاملMicrobial community composition and function across an arctic tundra landscape.
Arctic landscapes are characterized by a diversity of ecosystems, which differ in plant species composition, litter biochemistry, and biogeochemical cycling rates. Tundra ecosystems differing in plant composition should contain compositionally and functionally distinct microbial communities that differentially transform dissolved organic matter as it moves downslope from dry, upland to wet, low...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2004
ISSN: 0168-6496,1574-6941
DOI: 10.1016/j.femsec.2004.05.003